Journal article
Direct demonstration of lipid phosphorylation in the lipid bilayer of the biomimetic bicontinuous cubic phase using the confined enzyme lipid A phosphoethanolamine transferase
L Van 't Hag, A Anandan, SA Seabrook, SL Gras, CJ Drummond, A Vrielink, CE Conn
Soft Matter | ROYAL SOC CHEMISTRY | Published : 2017
DOI: 10.1039/C6SM02487D
Abstract
Retention of amphiphilic protein activity within the lipid bilayer membrane of the nanostructured biomimetic bicontinuous cubic phase is crucial for applications utilizing these hybrid protein-lipid self-assembly materials, such as in meso membrane protein crystallization and drug delivery. Previous work, mainly on soluble and membrane-associated enzymes, has shown that enzyme activity may be modified when immobilized, including membrane bound enzymes. The effect on activity may be even greater for amphiphilic enzymes with a large hydrophilic domain, such as the Neisserial enzyme lipid A phosphoethanolamine transferase (EptA). Encapsulation within the biomimetic but non-endogenous lipid bila..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
We thank Drs Adrian M. Hawley, Stephen T. Mudie, Nigel M. Kirby and Timothy M. Ryan for their assistance with SAXS experiments, and we acknowledge use of the SAXS/WAXS beamline at the Australian Synchrotron. We thank Dr Janet Newman for help with the robotic setup of plates at the C3 Collaborative Crystallization Centre, CSIRO, Parkville, Australia. We acknowledge Dr Ching-Seng Ang for help with RP-HPLC and ESI-TOF MS measurements. Ms Leonie van't Hag is supported by a Melbourne International Research Scholarship and a CSIRO PhD scholarship. Ms Anandhi Anandan acknowledges support through a Fay Gale Fellowship from the University of Western Australia. A/Prof. Sally L. Gras is supported by the ARC Dairy Innovation Hub IH120100005. Funding support to Prof. Alice Vrielink from the National Health and Medical Research Council of Australia is acknowledged (APP1003697 and APP1078642). Dr Charlotte Conn is the recipient of an ARC DECRA Fellowship DE160101281.